> The Nanopore tools evolve fast, and most of them are not under a Free > license, but the following might be useful: I am not sure how the tools are used with Nanopore sequencing in the Nextflow pipeline, how about just using alternative tools under a free license? Such as https://github.com/ocxtal/minialign https://github.com/jts/nanopolish https://github.com/lh3/minimap https://github.com/lh3/miniasm -- Jun | He - His - Him