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Re: How to convert gb to gbk to get proper input for mauvealigner?



Hi, Andreas,

On الخميس 10 آذار 2016 09:05, Andreas Tille wrote:
> Hi,
> 
> a colleague of mine relaised that mauvealigner seems to need gbk files
> when running under Linux (while under Win also gb file is working).  This
> happens when using
> 
>    java -Xmx6g -cp /usr/share/java/Mauve.jar org.gel.mauve.contigs.ContigOrderer -output OUTPUTDIR -ref REFERENCE.gbk -draft DRAFT.contigs.fasta
> 
> While under win also REFERENCE.gb is OK this leads to an error under
> (the Debian packaged!) mauvealigner (I forgot to ask what version is
> used under Win - may be there is a difference as well).
>

As far as I know, gb and gbk are interchangeable extensions for the
genbank format. The genbank format may or may not include the actual
sequence data. If you're passing a genbank file to Mauve, it should be
including the sequence data.


> The solution of my colleague was to use CloneManager under Windows to do
> a gb -> gbk conversion.  Is there any tool in our rich toolset which can
> be used here to script this?
> 

I'm not aware of any such tools; I've never needed to convert from one
to the other since I usually get a reference sequence from NCBI, where I
can download either type of genbank file directly.

regards
Afif

-- 
Afif Elghraoui | عفيف الغراوي
http://afif.ghraoui.name


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